Ex vivo expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience)
Abstract
Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The improvement of MSC expansion standard protocols allowing receiving necessary MSC amount with the ability of self-renewal and multilineage differentiation potential for clinical application is required. The present article summarizes the current experience concerning uses of various culture mediums and supplements for the ex vivo MSC expansion. The author's experience of ex vivo MSC expansion using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of medium type on MSC growth and immunophenotype is analyzed.
About the Authors
T. V. Shamanskaya
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public health
Russian Federation
Russian Federation
The Stem cell bank
Moscow
Ye. Yu. Osipova
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public health
Russian Federation
Russian Federation
The Stem cell bank
Moscow
B. B. Purbueva
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public health
Russian Federation
Russian Federation
The Stem cell bank
Moscow
A. Yu. Ustyugov
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology
Russian Federation
Russian Federation
Moscow
T. A. Astrelina
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public health
Russian Federation
Russian Federation
The Stem cell bank
Moscow
M. V. Yakovleva
Moscow Department of Public health
Russian Federation
Russian Federation
The Stem cell bank
Moscow
S. A. Rumyantsev
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology
Russian Federation
Russian Federation
Moscow
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Review
For citations:
Shamanskaya T.V., Osipova Ye.Yu., Purbueva B.B., Ustyugov A.Yu., Astrelina T.A., Yakovleva M.V., Rumyantsev S.A. Ex vivo expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience). Oncohematology. 2010;(3):65-71. (In Russ.)
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