Results of external quality control study in flow cytometric acute lymphoblastic leukemia diagnostics
https://doi.org/10.17650/1818-8346-2016-11-3-68-75
Abstract
Comparison of interpretation of acute lymphoblastic leukemia (ALL) flow cytometric diagnostics data was the aim of the study. Immunophenotyping data obtained from 10 patients with ALL were analysed separately in 26 laboratories from Russian Federation and Kazahstan. Results comparison showed four main type of discordance: B-lineage ALL diagnostics during heavy bone marrow regeneration, great variability of T-ALL interpretation, complexity of ambiguous lineage acute leukemia and, finally, very different report types, unique for each laboratory. All these problems are the serious obstacles for standardization of flow cytometric ALL diagnostics in multicenter setting. Continuation of similar QC rounds following by consecutive discussions with further development of consensus diagnostic algorithm could be the first step for standardization of ALL immunophenotyping in Russian Federation and CIS countries.
About the Authors
A. M. Popov
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia
Russian Federation
Russian Federation
T. U. Verzhbitskaya
Regional Children Clinical Hospital No 1
Russian Federation
Russian Federation
E. E. Zueva
Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg
State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
O. V. Anan’eva
Tyumen State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
E. V. Babenko
I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, R. M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantology
Russian Federation
Russian Federation
L. V. Baydun
Russian Children Clinical Hospital, Ministry of Health of Russia
Russian Federation
Russian Federation
E. A. Belyakova
North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of Russia
Russian Federation
Russian Federation
E. V. Boyakova
Blood Transfusion Station of the Moscow Healthcare Department
Russian Federation
Russian Federation
O. G. Bortnikova
Regional Children Clinical Hospital
Russian Federation
Russian Federation
M. V. Gorchakova
Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
L. Yu. Grivtsova
N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
Russian Federation
Russian Federation
E. N. Grinkevich
Nizhnevartovsk Regional Children Clinical Hospital
Russian Federation
Russian Federation
Yu. V. Davydova
Nizhnevartovsk Regional Children Clinical Hospital
Russian Federation
Russian Federation
E. I. Zakhar’ko
Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia
Russian Federation
Russian Federation
O. I. Illarionova
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia
Russian Federation
Russian Federation
N. M. Kaprano
Scientific Clinical Laboratory of Immunophenotyping of Blood Cells and Bone Marrow, National Research Center for Hematology, Ministry of Health of Russia
Russian Federation
Russian Federation
E. A. Koroleva
Diagnostic Center of the Altai Region
Russian Federation
Russian Federation
S. A. Kochengina
Chelyabinsk Regional Children Clinical Hospital
Russian Federation
Russian Federation
E. G. Kuz’mina
A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia
Russian Federation
Russian Federation
E. A. Kustova
Research Center of Pediatrics and Pediatric Surgery
Kazakhstan
Kazakhstan
T. A. Makarova
Children City Hospital No 1
Russian Federation
Russian Federation
Yu. V. Mirolyubova
ФГБУ «Северо-Западный федеральный медицинский исследовательский центр» Минздрава России
Russian Federation
Russian Federation
O. E. Murashkina
Children Regional Clinical Hospital
Russian Federation
Russian Federation
T. Yu. Mushkarina
A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center,
Ministry of Health of Russia
Russian Federation
Russian Federation
E. S. Nisheva
Children City Hospital No 1
Russian Federation
Russian Federation
I. A. Novikova
Rostov Research Institute of Oncology, Ministry of Health of Russia
Russian Federation
Russian Federation
E. Yu. Osipova
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia
Russian Federation
Russian Federation
G. E. Pluzhnikova
Regional Children Clinical Hospital
Russian Federation
Russian Federation
M. E. Pochtar
Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
Russian Federation
Russian Federation
N. V. Pronkina
Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Russian Federation
Russian Federation
E. V. Rusanova
Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
E. B. Rybkina
Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia
Russian Federation
Russian Federation
O. V. Seliverstova
Blood Transfusion Station of the Moscow Healthcare Department
Russian Federation
Russian Federation
N. N. Tupitsin
N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
Russian Federation
Russian Federation
G. I. Uleyskaya
City Clinical Hospital No 31
Russian Federation
Russian Federation
N. T. Urazalieva
Research Center of Pediatrics and Pediatric Surgery
Kazakhstan
Kazakhstan
L. G. Fechina
Regional Children Clinical Hospital No 1
Russian Federation
Russian Federation
O. V. Khoroshikh
Irkutsk the Badge of Honor Order Regional Clinical Hospital
Russian Federation
Russian Federation
O. E. Tsareva
Saratov State Medical University named after V.I. Razumovsky, Ministry of Health of Russia
Russian Federation
Russian Federation
L. A. Shchekina
City Clinical Hospital No 31
Russian Federation
Russian Federation
S. A. Plyasunova
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia
Russian Federation
Russian Federation
S. A. Lugovskaya
Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
Russian Federation
Russian Federation
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Review
For citations:
Popov A.M., Verzhbitskaya T.U., Zueva E.E., Anan’eva O.V., Babenko E.V., Baydun L.V., Belyakova E.A., Boyakova E.V., Bortnikova O.G., Gorchakova M.V., Grivtsova L.Yu., Grinkevich E.N., Davydova Yu.V., Zakhar’ko E.I., Illarionova O.I., Kaprano N.M., Koroleva E.A., Kochengina S.A., Kuz’mina E.G., Kustova E.A., Makarova T.A., Mirolyubova Yu.V., Murashkina O.E., Mushkarina T.Yu., Nisheva E.S., Novikova I.A., Osipova E.Yu., Pluzhnikova G.E., Pochtar M.E., Pronkina N.V., Rusanova E.V., Rybkina E.B., Seliverstova O.V., Tupitsin N.N., Uleyskaya G.I., Urazalieva N.T., Fechina L.G., Khoroshikh O.V., Tsareva O.E., Shchekina L.A., Plyasunova S.A., Lugovskaya S.A. Results of external quality control study in flow cytometric acute lymphoblastic leukemia diagnostics. Oncohematology. 2016;11(3):68-75. (In Russ.) https://doi.org/10.17650/1818-8346-2016-11-3-68-75
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