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Comparative analysis of minor histocompatibility antigens genotyping methods
- Authors: Vdovin A.S.1,2, Postovskaya A.M.1,3,4, Bykova N.A.1,2, Romaniuk D.S.1,2, Alieva A.K.5,6, Yefimova P.R.1,3,4, Sheetikov S.A.7,4, Julhakyan H.L.1,2, Efimov G.A.1,2
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Affiliations:
- National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation
- 4 Novy Zyikovskiy pr., Moscow, 125167, Russia
- 4 Novy Zyikovskiy pr., Moscow, 125167, Russia M.V. Lomonosov Moscow State University
- 1 Leninskie Goryi St., Moscow, 119991, Russia
- The Institute of Molecular Genetics of the Russian Academy of Sciences
- 2, Kurchatova Sq., Moscow, 123182, Russia
- M.V. Lomonosov Moscow State University
- Issue: Vol 11, No 2 (2016)
- Pages: 40-50
- Section: BASIC RESEARCH
- Published: 05.08.2016
- URL: https://oncohematology.abvpress.ru/ongm/article/view/196
- DOI: https://doi.org/10.17650/1818-8346-2016-11-2-40-50
- ID: 196
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Abstract
The wide range of techniques could be employed to find mismatches in minor histocompatibility antigens between transplant recipients and their donors. In the current study we compared three genotyping methods based on polymerase chain reaction (PCR) for four minor antigens. Three of the tested methods: allele-specific PCR, restriction fragment length polymorphism and real-time PCR with TaqMan probes demonstrated 100% reliability when compared to Sanger sequencing for all of the studied polymorphisms. High resolution melting analysis was unsuitable for genotyping of one of the tested minor antigens (HA-1) as it has linked synonymous polymorphism. Obtained data could be used to select the strategy for large-scale clinical genotyping.
About the authors
A. S. Vdovin
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaRussian Federation
A. M. Postovskaya
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaM.V. Lomonosov Moscow State University; 1 Leninskie Goryi St., Moscow, 119991, Russia
Russian Federation
N. A. Bykova
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaRussian Federation
D. S. Romaniuk
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaRussian Federation
A. K. Alieva
The Institute of Molecular Genetics of the Russian Academy of Sciences; 2, Kurchatova Sq., Moscow, 123182, RussiaRussian Federation
P. R. Yefimova
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaM.V. Lomonosov Moscow State University; 1 Leninskie Goryi St., Moscow, 119991, Russia
Russian Federation
S. A. Sheetikov
M.V. Lomonosov Moscow State University; 1 Leninskie Goryi St., Moscow, 119991, RussiaRussian Federation
H. L. Julhakyan
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, RussiaRussian Federation
G. A. Efimov
National Research Center for Hematology of the Ministry of Healthcare of the Russian Federation; 4 Novy Zyikovskiy pr., Moscow, 125167, Russia
Author for correspondence.
Email: grigory@efimov.info
Russian Federation
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