Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation
https://doi.org/10.17650/1818-8346-2011-6-3-76-81
Abstract
Phase of incubation microarray with cell suspension is prolonged when cells are investigated. It takes from 20 to 60 min if cell sedimentation on the surface of microarray is the result of gravity . Decrease of this stage duration is possible due to centrifugation. In th is article influence of centrifugation on results of analysis is considered. Changes of morphological description of cells are estimated when they a re precipitated
with different acceleration. Also availability of centrifugation using when it is necessary to obtain the high density of cell binding in test regions of microarray if cells concentration in sample is small is demonstrated.
About the Authors
A. V. Shishkin
Izhevsk State Medical Academy
Russian Federation
Russian Federation
N. G. Ovchinina
Izhevsk State Medical Academy
Russian Federation
Russian Federation
S. S. Bessmeltsev
Russian Research Institute of Hematology and Transfusiology
Russian Federation
Russian Federation
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Review
For citations:
Shishkin A.V., Ovchinina N.G., Bessmeltsev S.S. Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation. Oncohematology. 2011;6(3):76-81. (In Russ.) https://doi.org/10.17650/1818-8346-2011-6-3-76-81
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