Determination of eosinophilic cationic protein and tryptase in graft versus host disease and hematological malignancies with eosinophilia
https://doi.org/10.17650/1818-8346-2008-0-4-26-30
Abstract
Diseases with eosinophilia the pathogenesis of which is unclear are of particular clinical significance among hematological malignancies. These include acute and chronic graft versus host disease (GVHD) associated with eosinophilia, hypereosinophilic syndrome and chronic myeloid leukemias, Hodgkin's and non-Hodgkin's lymphomas. The role of eosinophils and mast cells intracellular proteins in proliferative diseases is unknown. The objective of the investigation was to study the significance of tryptase and eosinophilic cationic protein (ECP) measurements in patients with chronic GVHD and hematological malignancies. Thirty two patients with verified oncohematological diseases (a study group) — 6 patients with chronic GVHD after allogeneic hemopoietic stem cells transplantation, 18 with asthma and 8 with solid tumors - were included in the study. The serum concentrations of tryptase and ECP were measured by immunofluorescence assay. Elevated ECP levels were found in patients with GVHD (p < 0.03) and in those with lymphoproliferative diseases (p = 0.007) as compared to the nonhematological group (p < 0.03). Increased tryptase level was recorded in patients with GVHD and lymphoproliferative diseases as compared to those with solid tumors and nonhematological disorders (p = 0.03), as well as in the GVHD versus lymphoproliferation groups (p < 0.05). A direct correlation between ECP concentration and the eosinophils count in patients with lymphoproliferative diseases (r = 0.9; p = 0.000001) was found. The authors have concluded that measurement of soluble eosinophilic proteins levels and mast cell enzymes is reasonable to diagnostics and monitoring of various hypereosinophilic states in patients with chronic GVHD and hematological malignancies.
About the Authors
L. S. Komarova
Saint-Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
Saint-Petersburg
N. B. Mikhailova
Saint-Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
Saint-Petersburg
A. A. Totolyan
Saint-Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
Saint-Petersburg
B. V. Afanasyev
Saint-Petersburg Pavlov State Medical University
Russian Federation
Russian Federation
Saint-Petersburg
References
1. Ellyard J.I., Simson L., Parish C.R. Th2-mediated anti-tumor immunity: friend or foe? J compilat 2007;70:1—11.
2. Фрейдлин И.С., Тотолян А.А. Клетки иммунной системы. СПб.: Наука, 2000. Т. 3—5.
3. McNeel D., Rubio M.T., Damaj G. Hypereosinophilia as a presenting sign of acute graft-versus-host disease after allogenic bone marrow transplantation. Transplantation 2002;74:1797.
4. Комарова Л.С., Зуева Е.Е., Нестерович И.И. и др. Роль внутриклеточных белков эозинофилов и тучных клеток в развитии эозинофилии при различных заболеваниях. Рос иммунол журн 2007;1(10):27—33.
5. Shakoory B., Fitzgerald S.M., Lee S.A. et al. The role of human mast cell derived cytokines in eosinophil biology. J Interferon and Cytokine Res 2004;24:271—81.
6. Hallgren J., Pejler G. Biology of mast cell tryptase: an inflammatory mediator. FEBS J 2006; 25:234—41.
7. Fletchera S., Bain B. Diagnosis and treatment of hypereosinophilic syndromes. Current Opinion in Hematology 2007;14(1):37—42.
8. Реброва О.Ю. Статистический анализ медицинских данных. Применение пакета прикладных программ STATISTICA. М.: МедиаСфера, 2003.
9. Ayalew T.A., Patnaik M.M., Pardanani A. Eosinophilia: secondary, clonal and idiopathic. Br J Haematol 2006;133:468—92.
10. Gotlib J., Cross N.C.P., Gilliland D.G. Eosinophilic disorders: molecular pathogenesis, new classification, and modern therapy. Best Pract Res Clin Haematol 2006;19(3):535—69.
11. Sade K., Mysels A., Levo Y. et al. Eosinophilia: A study of 100 hospitalized patients. Eur J Intern Med 2007;18:196—201.
12. Ringden O. Immunotherapy by allogeneic stem cell transplantation. Adv Cancer Res 2007;97:25—60.
13. Jacobsohn D.A., Schechter T., Seshadri R. et al. Eosinophillia correlates with the presence or development of chronic graft-versus-host disease in children. Transplantation 2004;77(7):1096—100.
14. Aisa Y., Mori T., Nakazato T. et al. Blood eosinophilia as a marker of favorable outcome after allogeneic stem cell transplantation. J compilat Eur Soc for Org Transpl 2007;20:761—70.
15. Lotfi R., Lee J.J., Lotze M.T. Eosinophilic granulocytes and damageassociated molecular pattern molecules (DAMPs). Role in the inflammatory response within tumors. J Immunother 2007;30:116—28.
16. Mingomataj E.C. Eosinophil-induced prognosis improvement of solid tumors could be enabled by their vesicle-mediated barrier permeability induction. Med Hypoth 2008;70(3):582—4.
17. Puxeddu I., Alian A., Piliponsky A.M. et al. Human peripheral blood eosinophils induce angiogenesis. Int J Biochem Cell Biol 2005;37:628—36.
18. Sperr W.R., Hauswirth A.W., Valent P. Tryptase a novel biochemical marker of acute myeloid leukemia. Leuk Lymph 2002;43(12):2257—61.
19. Carvalho R.F.S., Mahshid Y., Claesson H.-E. et al. Expression of mast cell tryptases in Hodgkin and reed-sternberg (HRS) cells. J compilat Scand J Immunol 2007;67:53—6.
20. Molin D., Edstrom A., Glimelius I. et al. Mast cell infiltration correlates with poor prognosis in Hodgkin's lymphoma. Br J Haematol 2002;119(1):122—4.
21. Molin D., Fischer M., Xiang Z. et al. Mast cells express functional CD30 ligand and are the predominant CD30 L-positive cells in Hodgkin's disease. Br J Haematol 2001;114:616—23.
22. Sperr W.R., Stehberger B., Wimazal F. Serum tryptase measurements in patients with myelodysplastic syndromes. Leuk Lymphoma 2002;43:1097—105.
Review
For citations:
Komarova L.S., Mikhailova N.B., Totolyan A.A., Afanasyev B.V. Determination of eosinophilic cationic protein and tryptase in graft versus host disease and hematological malignancies with eosinophilia. Oncohematology. 2008;(4):26-30. (In Russ.) https://doi.org/10.17650/1818-8346-2008-0-4-26-30
Views:
315
Email this article 