RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS
https://doi.org/10.17650/1818-8346-2018-13-1-73-82
Abstract
Background. Flow cytometry is one of the key technologies for acute lymphoblastic leukemia (ALL) diagnostics. Nevertheless lack of technological standards hampers implementation of immunophenotyping data in treatment protocols.
Objective: development of harmonized guidelines for flow cytometric diagnostics of childhood ALL.
Materials and methods. Three reference laboratories of the immunophenotyping group “Moscow–Berlin” took part in the development and standardization of approaches to the cytometric determination of the minimal residual disease. The sequence of steps for staining with monoclonal antibodies, selection of reagents for immunophenotyping, flow cytometer adjustment algorithms were discussed.
Results. We developed and implemented in multicenter setting the harmonized approach for immunophenotyping flow cytometric childhood ALL. Successful integration of this protocol in the multicenter group has shown good level of our approach reproducibility.
Conclusion. These results will allow implementing diagnostic standards in stratification system of pediatric ALL treatment protocols of russianbelarusian multicenter group.
About the Authors
I. A. Novikova
D. Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
Russian Federation
1 Samory Mashela St., GSP-7, 117198 Moscow
Russian Federation
1 Samory Mashela St., GSP-7, 117198 Moscow
T. Yu. Verzhbitskaya
Pediatric Oncology/Hematology Center, Regional Children Hospital № 1;
Research Institute of Medical Cell Technologies
Russian Federation
32 Serafimy Deryabinoy St., 620149 Yekaterinburg, 22a Karla Marksa St., 620026 Yekaterinburg
Russian Federation
32 Serafimy Deryabinoy St., 620149 Yekaterinburg, 22a Karla Marksa St., 620026 Yekaterinburg
L. V. Movchan
Belarussian Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
43 Frunzenskaya St., Borovlyani, 223053 Minsk region
Belarus
43 Frunzenskaya St., Borovlyani, 223053 Minsk region
G. A. Tsaur
Pediatric Oncology/Hematology Center, Regional Children Hospital № 1;
Research Institute of Medical Cell Technologies
Russian Federation
32 Serafimy Deryabinoy St., 620149 Yekaterinburg, 22a Karla Marksa St., 620026 Yekaterinburg
Russian Federation
32 Serafimy Deryabinoy St., 620149 Yekaterinburg, 22a Karla Marksa St., 620026 Yekaterinburg
М. V. Belevtsev
Belarussian Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
43 Frunzenskaya St., Borovlyani, 223053 Minsk region
Belarus
43 Frunzenskaya St., Borovlyani, 223053 Minsk region
A. M. Popov
D. Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
Russian Federation
1 Samory Mashela St., GSP-7, 117198 Moscow
Russian Federation
1 Samory Mashela St., GSP-7, 117198 Moscow
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Review
For citations:
Novikova I.A., Verzhbitskaya T.Yu., Movchan L.V., Tsaur G.A., Belevtsev М.V., Popov A.M. RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS. Oncohematology. 2018;13(1):73-82. (In Russ.) https://doi.org/10.17650/1818-8346-2018-13-1-73-82
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