OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press756BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕEx vivo expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience)Культивирование мезенхимальных стволовых клеток Ex vivo в различных питательных средах (обзор литературы и собственный опыт)ShamanskayaT. V.ШаманскаяТ. В.
Russian Federation

The Stem cell bank

Moscow

Татьяна Викторовна Шаманская

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

ГУЗ Банк стволовых клеток

Москва

tottilll@list.ruOsipovaYe. Yu.ОсиповаЕ. Ю.
Russian Federation

The Stem cell bank

Moscow

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

ГУЗ Банк стволовых клеток

Москва

PurbuevaB. B.ПурбуеваБ. Б.
Russian Federation

The Stem cell bank

Moscow

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

ГУЗ Банк стволовых клеток

Москва

UstyugovA. Yu.УстюговА. Ю.
Russian Federation

Moscow

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

Москва

AstrelinaT. A.АстрелинаТ. А.
Russian Federation

The Stem cell bank

Moscow

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

ГУЗ Банк стволовых клеток

Москва

YakovlevaM. V.ЯковлеваМ. В.
Russian Federation

The Stem cell bank

Moscow

ГУЗ Банк стволовых клеток

Москва

RumyantsevS. A.РумянцевС. А.
Russian Federation

Moscow

ФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии

Москва

Federal Clinical Research Center of Pediatric Hematology, Oncology and ImmunologyМинздравсоцразвития РоссииMoscow Department of Public healthДепартамент Здравоохранения260820103657126112022261120222010https://oncohematology.abvpress.ru/ongm/article/view/756

   Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The improvement of MSC expansion standard protocols allowing receiving necessary MSC amount with the ability of self-renewal and multilineage differentiation potential for clinical application is required. The present article summarizes the current experience concerning uses of various culture mediums and supplements for the ex vivo MSC expansion. The author's experience of ex vivo MSC expansion using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of medium type on MSC growth and immunophenotype is analyzed.

   В последнее время мезенхимальные стволовые клетки (МСК) нашли широкое применение во многих областях медицины, где используются их иммуносупрессивные свойства, влияние на процессы регенерации поврежденных тканей и т. д. В связи с этим возникает потребность в разработке стандартизированных протоколов экспансии МСК, которые позволят получить необходимое количество клеток для клинического применения без нарушения их способности к самовозобновлению и мультилинейного дифференцированного потенциала. В настоящей статье обобщены данные литературы о различных питательных средах и добавках, применяемых при культивировании ex vivo МСК. Представлен собственный опыт использования стандартной питательной среды для культивирования МСК ДМЕМ с добавлением эмбриональной телячьей сыворотки и бессывороточной питательной среды MesenCult MSC Basal Medium (Human) (StemCell technologies Inc) с добавлением Mesenchymal Stem Cell Stimulatory Supplements (Human). Проведена оценка их влияния на рост и иммунофенотипические характеристики МСК.

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