OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press6210.17650/1818-8346-2012-7-2-51-56BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕMolecular cytogenetics for acute megakaryocytic leukemia diagnosisМетоды молекулярной цитогенетики для диагностики острого мегакариобластного лейкозаMatveevaE. A.МатвееваЕ. А.
Russian Federation
KazakovaA. N.КазаковаА. Н.
Russian Federation
KalininaI. I.КалининаИ. И.
Russian Federation
DubrovinaM. E.ДубровинаМ. Э.
Russian Federation
BaydunL. V.БайдунЛ. В.
Russian Federation
MaschanM. A.МасчанМ. А.
Russian Federation
OlshanskayaYu. V.ОльшанскаяЮ. В.
Russian Federation
yuliaolshanskaya@gmail.comMaschanA. A.МасчанА. А.
Russian Federation
D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and ImmunologyФГБУ ФНКЦ ДГОИ им. Дмитрия Рогачева Минздрава РоссииRussian Children Clinical HospitalФГУ «Российская детская клиническая больница» Минздрава России2205201272515622072014220720142012https://oncohematology.abvpress.ru/ongm/article/view/62

Acute megakaryocytic leukemia (AML M7) – a rare disease characterized by poor treatment response, except for t(1;22) variant in infants. Cytogenetic abnormalities in AML M7 are highly heterogeneous. We collected samples from children with AML M7 to analyze the disease cytogenetic profile. During September 2009 to March 2012 20 AML M7 patients was studied using fluorescence in situ hybridization. Complex and heterogeneous chromosomal abnormalities were revealed. It was found that no recurring abnormalities and cytogenetic markers unique to each patients. Also, the 19p13 amplification described previously only in myeloid cell lines was detected.

Острый мегакариобластный лейкоз (ОМЛ М7) – редкое заболевание, характеризующееся плохим ответом на лечение, за исключением варианта с t(1;22) у детей до года. Цитогенетические перестройки при ОМЛ М7 отличаются высокой гетерогенностью. В нашем исследовании мы собрали случаи ОМЛ М7 у детей, чтобы определить цитогенетический профиль этого заболевания. За период с сентября 2009 по март 2012 г. нами с помощью методов флуоресцентной in situ гибридизации было исследовано 20 больных ОМЛ М7. Был показан комплексный и гетерогенный характер хромосомных перестроек. Оказалось, что повторяющиеся перестройки практически отсутствуют и цитогенетические маркеры уникальны для каждого пациента. Также обнаружено явление амплификации 19р13, описанное ранее только в миелоидных клеточных линиях.

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