OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press61210.17650/1818-8346-2008-0-1-2-6-12ФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯMicroarrays: a new era in development of oncohematologyМикрочипы: новый этап в развитии онкогематологииNikitinYe. A.НикитинЕ. А.
Russian Federation

Москва

SudarikovA. B.СудариковА. Б.
Russian Federation

Москва

BaranovaA. V.БарановаА. В.
Russian Federation

Москва

Research Center for Hematology of Russian Academy of Medical SciencesГематологический научный центр РАМНMedical Genetic Research Center of Russian Academy of Medical SciencesМедико-генетический научный центр РАМН230520081-261222112022221120222008https://oncohematology.abvpress.ru/ongm/article/view/612

Introduction of the immunophenotyping in 70-ties and 80-ties of the past century has led to significant revision of conception of hematological malignancies. A new era in developement of medicine and oncology in particular is related to functional genomics. This field of molecular biology attempts to make use of the vast wealth of data produced by genomic projects for studying gene functions and interactions. Functional genomics focus on the dynamic aspects such as gene transcription, mRNA translation, and protein-protein interactions, as opposed to the static aspects of the genomic information such as DNA sequence or structure. Several genomic technologies have been developed to study gene expression. Gene expression profiling on DNA microarrays has been particularly useful in analyzing expression of thousands genes in parallel. Modern microarrays contain comprehensive probe sets which are able to measure the majority of genes encoded in human genome. Here we focus on microarray technology, discuss its clinical applications and briefly review some results obtained by this method in researches of hematological malignancies.

Появление иммунофенотипирования в 70—80-х годах прошлого века привело к радикальному изменению представлений о гемобластозах, к созданию более совершенной классификации. Новой вехой в развитии медицины, и в частности онкогематологии, стала функциональная геномика. Задача этого раздела молекулярной гематологии — изучение функций новых генов и генных сетей в нормальном развитии и жизнедеятельности тканей, а также при различных заболеваниях. Информация, полученная в ходе расшифровки генома человека, носит статичный характер. Функциональная геномика изучает динамические аспекты функционирования генов, такие как транскрипция, трансляция мРНК, белковые взаимодействия. Одним из методов функциональной геномики является технология биологических микрочипов, позволяющая одновременно анализировать уровень активности тысяч генов в одном эксперименте, причем масштаб измерений может приближаться к геномному. В данном обзоре мы рассматриваем технологию микрочипов, ее клинические приложения и кратко суммируем результаты экспериментов с микрочипами при лимфатических опухолях.

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