OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press4110.17650/1818-8346-2013-8-1-33-39HEMATOLOGIC MALIGNANCIES: DIAGNOSIS, TREATMENT, SUPPORTIVE CAREГЕМОБЛАСТОЗЫ: ДИАГНОСТИКА, ЛЕЧЕНИЕ, СОПРОВОДИТЕЛЬНАЯ ТЕРАПИЯImmunophenotypic investigation of infant acute myeloid leukemiaИммунофенотипическая характеристика острого миелоидного лейкоза у детей первого года жизниPopovA. M.ПоповА. М.
Russian Federation
uralcytometry@gmail.comTsaurG. A.ЦаурГ. А.
Russian Federation
VerzhbitskayaT. Yu.ВержбицкаяТ. Ю.
Russian Federation
StrenevaO. V.СтреневаО. В.
Russian Federation
ShorikovE. V.ШориковЕ. В.
Russian Federation
SavelievL. I.СавельевЛ. И.
Russian Federation
FechinaL. G.ФечинаЛ. Г.
Russian Federation
Regional Childrenʼs Clinical Hospital №1 Research Institute of Medical Cell TechnologiesГБУЗ СО ОДКБ № 1 ГБУЗ СО «Институт медицинских клеточных технологий»Regional Childrenʼs Clinical Hospital №1 Research Institute of Medical Cell Technologies Ural State Medical AcademyГБУЗ СО ОДКБ № 1 ГБУЗ СО «Институт медицинских клеточных технологий» ГБОУ ВПО УГМА2202201381333922072014220720142013https://oncohematology.abvpress.ru/ongm/article/view/41

Aim of the study – characterization of immunophenotype in infant acute myeloid leukemia (AML). 90 patients (40 boys and 50 girls) with acute leukemia (AL) aged up to 365 days were included in the current study. AML was found more frequently in infants than in older children (26.67 % and 10.83 % respectively; p = 0.0002). Significant immunophenotypic differences were observed in patients with and without MLL gene rearrangements. Number of cases in those tumor cells expressed CD99, CD61, CD133, CD15, NG2 varied between MLL-positive and MLL-negative groups. CD61-negativity, high CD99, CD15, CD133 and NG2 expression were immunophenotypic signatures of MLLrearranged infant AML, although CD99 and NG2 had the highest diagnostic efficacy. Thus infants’ AML immunophenotype differs significantly due to the presence of MLL gene rearrangements. Diagnostic immunophenotyping of infants’ AML allows predicting presence of MLL rearrangements by either CD99 or NG2 expression.

Цель исследования – характеристика иммунофенотипа острого миелоидного лейкоза (ОМЛ) у детей первого года жизни. В исследование было включено 90 пациентов (40 мальчиков и 50 девочек) с острым лейкозом (ОЛ) в возрасте от 0 до 11 месяцев включительно. Частота выявления ОМЛ составила 26,67 % от всех ОЛ у детей первого года жизни, что было значительно чаще, чем у более старших детей (10,83 %; р = 0,0002). Отсутствие экспрессии CD61, а также высокая экспрессия CD99, CD15, CD133 и NG2 характеризовали иммунофенотип опухолевых клеток при наличии перестроек гена MLL. Диагностическая эффективность выявления экспрессии CD99 и NG2 для прогнозирования наличия перестроек гена MLL оказалась наиболее высокой. Таким образом, иммунофенотип ОМЛ у детей младше 1 года существенно различается в зависимости от наличия или отсутствия перестроек гена MLL. Описанные особенности экспрессии антигенов позволяют прогнозировать наличие какой-либо перестройки гена MLL при ОМЛ у детей первого года жизни, при этом наибольшей диагностической эффективностью обладает наличие экспрессии CD99 или NG2.

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