OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press34910.17650/1818-8346-2019-14-2-59-69BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕEx vivo-model design and evaluation of the sensitivity of blast cells to chemotherapy as a way to personalize the treatment of acute myeloid leukemiaРазработка ex vivo-модели и оценка химиочувствительности бластных клеток в индивидуализации терапии острых миелоидных лейкозовhttps://orcid.org/0000-0001-9238-8476PolyakovA. S.ПоляковА. С.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

doctorpolyakov@gmail.comhttps://orcid.org/0000-0003-1213-8472NoskovYa. A.НосковЯ. А.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0001-7892-2783NikitinYu. V.НикитинЮ. В.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0002-0470-1109TyrenkoV. V.ТыренкоВ. В.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0003-2441-9394KolubaevaS. N.КолюбаеваС. Н.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0003-1964-3690BogdanovA. N.БогдановА. Н.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0002-9319-462XSemelevV. N.СемелевВ. Н.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

PetrovaO. R.ПетроваО. Р.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0003-2990-8621BondarchukS. V.БондарчукС. В.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0002-6715-0340ZhogolevD. K.ЖоголевД. К.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0003-4918-1976ZolotarevA. D.ЗолотарёвА. Д.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0001-5884-2057KovalevA. V.КовалевА. В.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

https://orcid.org/0000-0002-0790-8746PuchkovaYu. E.ПучковаЮ. Е.
Russian Federation

6 Akademika Lebedeva St., Saint-Petersburg, 194044 Russia

Россия, 194044 Санкт-Петербург, ул. Академика Лебедева, 6

Military Medical Academy named after S.M. Kirov, Ministry of Defense of the RussiaФГБВОУ ВО «Военно-медицинская академия им. С.М. Кирова» Минобороны России2607201914259692507201925072019https://oncohematology.abvpress.ru/ongm/article/view/349

Despite continuous attempts to improve therapy, the outcomes of acute myeloid leukemia remain almost unchanged over last decades. Drugs made with a more complete understanding of the biology of acute myeloid leukemia do not equal the hopes for better prognosis. The best results are achieved only with high-dose chemotherapy, which is only possible for a limited number of patients. High phenotypic and genotypic heterogeneity of acute myeloid leukemia defines the relevance to develop personalized approaches to therapy, including those based on determination of individual drug sensitivity of blast cells.This article presents the results of developing an ex-vivo model of acute myeloid leukemia, as well as testing of two in vitro sensitivity assessment methods: evaluation of the genotoxicity of drugs in the micronucleus test and vitality and sensitivity to chemotherapy in sorted blast cells. Prospects of individualized therapy of acute myeloid leukemia were determined based on introduction into clinical practice and continuing the research.

Несмотря на постоянные попытки совершенствования терапии, исходы острых миелоидных лейкозов остаются практически неизменными уже на протяжении нескольких десятилетий. Пока не оправдывают надежд на изменение ситуации даже перспективные препараты, создаваемые с учетом более глубокого понимания биологии лейкозов. По-прежнему наилучшие результаты достигаются лишь при высокодозной индукционной химиотерапии, применение которой возможно только у ограниченного числа пациентов. Высокая фенотипическая и генотипическая разнородность миелоидных лейкозов определяет актуальность развития персонифицированных подходов к терапии, в том числе основанных на определении индивидуальной химиочувствительности опухолевых клеток.В статье представлены результаты разработки модели острого миелоидного лейкоза ex vivo, а также апробации 2 методов исследования химиочувствительности in vitro: оценки генотоксичности противоопухолевых препаратов в микроядерном тесте, а также жизнеспособности и химиочувствительности отсортированных бластных клеток. Определены перспективы индивидуализированной терапии миелоидных лейкозов на основании внедрения и дальнейшего совершенствования результатов работы.

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