OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press23110.17650/1818-8346-2017-12-1-55-61BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕImmunophenotypic features of bone marrow tumor cell in Burkitt lymphoma/leukemia: B-lineage acute lymphoblastic leukemia diagnostics opportunitiesИммунофенотипическая характеристика опухолевых клеток в костном мозге при лимфоме/лейкозе Беркитта: возможности дифференциальной диагностики с острым лимфобластным лейкозомDeminaI. A.ДёминаИ. А.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

VerzhbitskayaT. Yu.ВержбицкаяТ. Ю.
Russian Federation

32 Serafimy Deryabinoy St., Ekaterinburg 620149, Russia;

22A Karlа Marksа St., Ekaterinburg 620026, Russia;

Россия, 620149 Екатеринбург, ул. Серафимы Дерябиной, 32;

Россия, 620026 Екатеринбург, ул. Карла Маркса, 22, корп. А

KashporS. A.КашпорС. А.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

PlyasunovaS. A.ПлясуноваС. А.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

DubrovinaM. E.ДубровинаМ. Э.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

FechinaL. G.ФечинаЛ. Г.
Russian Federation

32 Serafimy Deryabinoy St., Ekaterinburg 620149, Russia;

22A Karlа Marksа St., Ekaterinburg 620026, Russia;

Россия, 620149 Екатеринбург, ул. Серафимы Дерябиной, 32;

Россия, 620026 Екатеринбург, ул. Карла Маркса, 22, корп. А

MyakovaN. V.МяковаН. В.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

SamochatovaE. V.СамочатоваЕ. В.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

MaschanA. A.МасчанА. А.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

PopovA. M.ПоповА. М.
Russian Federation

1 Samory Mashela St., Moscow 117997, Russia;

Россия, 117997 Москва, ул. Саморы Машела, 1;

uralcytometry@gmail.comNational Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of RussiaФГБУ «Национальный научно-практический центр детской гематологии, онкологии и иммунологии им. Дмитрия Рогачева» Минздрава РоссииSverdlovsk Regional Clinical Children’s Hospital No 1ГБУЗ Свердловской области «Областная детская клиническая больница № 1»Institute of Medical Cell TechnologiesГАУЗ Свердловской области «Институт медицинских клеточных технологий»2604201712155612604201726042017https://oncohematology.abvpress.ru/ongm/article/view/231

Bone marrow tumor blasts immunophenotyping is an essential part of Burkitt lymphoma/leukemia (BL) and B-cell precursor acute lymphoblastic leukemia (BCP-ALL) differential diagnostics. Nevertheless immunoglobulin heavy and light chains detection on the cell surface could meet several biological and methodological pitfals. Thus the aim of the present study was development of additional BL immunophenotypic criteria. Leukemic blasts antigen profile in 21 BL cases and 84 children with BCP-ALL was compared in a retrospective way. Antigen expression patterns in BL and BCP-ALL were significantly different. It was shown that even in cases with weak immunoglobulin M expression these two tumor types could be distinguished well by complex immunophenotype analysis. In present study all cases of CD34-negative B-lineage ALL without myeloid coexpression and with high CD20-positive cells proportion belonged to BL.

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