OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press14510.17650/1818-8346-2015-10-2-51-57BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕOral microflora in children with hematologic malignanciesМикрофлора ротовой полости де тей с онкогематологическими заболеваниямиVecherkovskayaM. F.ВечерковскаяМ. Ф.
Russian Federation
TetsG. V.ТецГ. В.
Russian Federation
AfanasievB. V.АфанасьевБ. В.
Russian Federation
TetsV. V.ТецВ. В.
Russian Federation
vtetzv@yahoo.comAcad. I.P. Pavlov First St. Petersburg State Medical University, Ministry of Health of RussiaГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Минздрава России6–8 L, va Tolstogo St., St. Petersburg, 197022, Russia197022, Россия, Санкт-Петербург, ул. Льва Толстого, 6–80406201510251570406201504062015https://oncohematology.abvpress.ru/ongm/article/view/145

The goal was a comprehensive study of oral microflora in healthy children and those with hematologic malignancies, based on the analysis of mixed microbial biofilms composition, isolation and identification of new previously unknown microorganisms. The material was obtained in children with hematological diseases in remission, 2–10 years aged, and for the control group from St. Petersburg schoolchildren and in kindergartens. We used microbiological, biochemical and molecular genetic methods, including electron microscopy, proteomic analysis, sequencing and complete genome annotation. Microorganisms of 23 genera isolated as pure cultures and identified by biochemical activity from mixed microbial biofilm derived from saliva of healthy and sick children. In microflora of children with hematologic malignancies a previously unknown type of streptococci with a large number of antibiotic resistance genes was revealed. Differences in oral microbiota composition of healthy children and children with hematological diseases in remission were revealed. The microbiota of children with hematologic malignancies contains more genes controlling antibiotic resistance. Also, it was observed previously unknown bacterium of the genus Streptococcus.

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