OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press10710.17650/1818-8346-2011-6-1-64-75BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕBiological properties of cord blood hematopoietic stem cellsБиологические свойства гемопоэтических стволовых клеток пуповинной кровиRumiantsevS. A.РумянцевС. А.
Russian Federation
s.roumiantsev@niidg.ruOsipovaE. Yu.ОсиповаЕ. Ю.
Russian Federation
IpatovS. E.ИпатовС. Е.
Russian Federation
ShamanskayaT. V.ШаманскаяТ. В.
Russian Federation
MayorovaO. A.МайороваО. А.
Russian Federation
RumyantsevA. G.РумянцевА. Г.
Russian Federation
Federal Research Center of Pediatric Hematology, Oncology and ImmunologyФГУ Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии Минздравсоцразвития России2402201161647524072014240720142011https://oncohematology.abvpress.ru/ongm/article/view/107

This article provides an overview of research evolution of umbilical cord blood as a source of hematopoietic stem cells for transplantation. Phased developments of the technology of stem cells detection and selection, their proliferation, differentiation and function assessment using cultivation in vitro and in vivo in animal model was described. Differences of cord blood stem cells in comparison with those from other sources were described. The role of microenvironment for stem cells self-renewal and differentiation is shown. The possibility of stem cells biological properties modeling on example of using a vector based on HIV type 1 containing Notch genes, to regulate CD133+ and CD34+ cord blood stem cells proliferation is shown.

Обзор литературы посвящен анализу эволюции изучения пуповинной крови как источника гемопоэтических стволовых клеток для трансплантации. Поэтапно описано развитие технологий определения и выделения стволовых клеток, оценки их способности к пролиферации и дифференцировке и функциональных характеристик с помощью методов культивирования in vitro и с помощью выращивания in vivo на животных моделях. Описаны различия стволовых клеток пуповинной крови в сравнении с другими тканевыми источниками. Показана роль микроокружения для самообновления и дифференцировки стволовых клеток. Исследованы возможности моделирования биологических свойств стволовых клеток на примере использования векторной конструкции на основе вируса иммунодефицита человека 1 типа, содержащей гены Notch, для регулирования пролиферации CD133+ и CD34+ гемопоэтических стволовых клеток пуповинной крови человека.

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