OncohematologyOncohematologyОнкогематология1818-83462413-4023Publishing House ABV Press101210.17650/1818-8346-2025-20-1-128-138BASIC RESEARCHФУНДАМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В ПРАКТИЧЕСКОЙ МЕДИЦИНЕ НА СОВРЕМЕННОМ ЭТАПЕMulticolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemiaИспользование многоцветной проточной цитометрии для диагностики макроглобулинемии Вальденстремаhttps://orcid.org/0000-0002-8490-6066GaltsevaI. V.ГальцеваИ. В.
Russian Federation

Irina Vladimirovna Glatseva

125167; 4 Novyy Zykovskiy Proezd; Moscow

Ирина Владимировна Гальцева

125167; Новый Зыковский пр-д, 4; Москва

galtseva.i@blood.ruhttps://orcid.org/0009-0005-7828-1556TsoyYu. A.ЦойЮ. А.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0003-4950-523XGrachevA. E.ГрачевА. Е.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0002-6512-910XKapranovN. M.КапрановН. М.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0002-4119-7175NikiforovaK. A.НикифороваК. А.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0001-5932-0285DavydovaYu. O.ДавыдоваЮ. О.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0009-0001-0544-6568KulikovA. A.КуликовА. А.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0002-2639-7419ZvonkovE. E.ЗвонковЕ. Е.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

https://orcid.org/0000-0001-6177-3566ParovichnikovaE. N.ПаровичниковаЕ. Н.
Russian Federation

125167; 4 Novyy Zykovskiy Proezd; Moscow

125167; Новый Зыковский пр-д, 4; Москва

National Medical Research Center for Hematology, Ministry of Health of RussiaФГБУ «Национальный медицинский исследовательский центр гематологии» Минздрава России190320252011281382103202521032025https://oncohematology.abvpress.ru/ongm/article/view/1012

   Waldenstrom macroglobulinemia is a lymphoplasmacytic lymphoma, the morphological substrates of which are b‑lymphocytes, proplasmocytes, and plasma cells. The world Health Organization recommends multicolor flow cytometry with analysis of markers such as IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103, Cd138, for diagnosing this disease. Based on international and our own experience, we recommend that tumor b‑lymphocytes and plasma cells be analyzed separately for the diagnosis of waldenstrom macroglobulinemia, since the immunophenotypic profile of these populations differs. In diagnostics, this approach provides a more complete understanding of various subpopulations contribution, and when monitoring minimal residual disease, it helps to detect the tumor clone, which after therapy is predominantly represented by plasma cells. we recommend using antibodies to surface and intracellular markers such as Cd138, Cd38, Cd19, Cd45, Cd20, Cd22, Cd27 cytκ, cytλ and cytIgM for immunophenotypic testing of waldenstrom macroglobulinemia.

   Макроглобулинемия Вальденстрема – лимфоплазмоцитарная лимфома, морфологическим субстратом которой являются В‑лимфоциты, проплазмоциты, а также плазматические клетки. Всемирная организация здравоохранения для диагностики данного заболевания рекомендует в клинической практике использовать метод многоцветной проточной цитометрии и анализировать такие маркеры, как IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103, Cd138. Опираясь на международный и собственный опыт, мы рекомендуем врачам анализировать отдельно опухолевые В‑лимфоциты и плазматические клетки при диагностике макроглобулинемии Вальденстрема, так как иммунофенотипический профиль этих популяций различается. В диагностике такой подход дает более полное представление о вкладе различных субпопуляций в опухолевую массу, а при мониторинге минимальной остаточной болезни помогает обнаружить опухолевый клон, который после терапии преимущественно представлен плазматическими клетками. Мы рекомендуем для иммунофенотипического исследования опухолевого субстрата при макроглобулинемии Вальденстрема использовать антитела к поверхностным и внутриклеточным маркерам, таким как Cd138, Cd38, Cd19, Cd45, Cd20, Cd22, Cd27, cytκ, cytλ и cytIgM.

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