Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation

Cover Page

Cite item

Full Text

Abstract

Phase of incubation microarray with cell suspension is prolonged when cells are investigated. It takes from 20 to 60 min if cell sedimentation on the surface of microarray is the result of gravity . Decrease of this stage duration is possible due to centrifugation. In th is article influence of centrifugation on results of analysis is considered. Changes of morphological description of cells are estimated when they a re precipitated
with different acceleration. Also availability of centrifugation using when it is necessary to obtain the high density of cell binding in test regions of microarray if cells concentration in sample is small is demonstrated.

About the authors

A. V. Shishkin

Izhevsk State Medical Academy

Author for correspondence.
Email: Shishkin_lab@mail.ru
Russian Federation

N. G. Ovchinina

Izhevsk State Medical Academy

Russian Federation

S. S. Bessmeltsev

Russian Research Institute of Hematology and Transfusiology

Russian Federation

References

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2011



СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77-36928 от  21.07.2009.