Results of external quality control study in flow cytometric acute lymphoblastic leukemia diagnostics

A. M. Popov; Попов А. М.; T. U. Verzhbitskaya; Вержбицкая Т. Ю.; E. E. Zueva; Зуева Е. Е.; O. V. Anan’eva; Ананьева О. В.; E. V. Babenko; Бабенко Е. В.; L. V. Baydun; Байдун Л. В.; E. A. Belyakova; Белякова Е. А.; E. V. Boyakova; Боякова Е. В.; O. G. Bortnikova; Бортникова О. Г.; M. V. Gorchakova; Горчакова М. В.; L. Yu. Grivtsova; Гривцова Л. Ю.; E. N. Grinkevich; Гринкевич Е. Н.; Yu. V. Davydova; Давыдова Ю. В.; E. I. Zakhar’ko; Захарько Е. И.; O. I. Illarionova; Илларионова О. И.; N. M. Kaprano; Капранов Н. М.; E. A. Koroleva; Королева Е. А.; S. A. Kochengina; Коченгина С. А.; E. G. Kuz’mina; Кузьмина Е. Г.; E. A. Kustova; Кустова Е. А.; T. A. Makarova; Макарова Т. А.; Yu. V. Mirolyubova; Миролюбова Ю. В.; O. E. Murashkina; Мурашкина О. Е.; T. Yu. Mushkarina; Мушкарина Т. Ю.; E. S. Nisheva; Нишева Е. С.; I. A. Novikova; Новикова И. А.; E. Yu. Osipova; Осипова Е. Ю.; G. E. Pluzhnikova; Плужникова Г. Э.; M. E. Pochtar; Почтарь М. Е.; N. V. Pronkina; Пронкина Н. В.; E. V. Rusanova; Русанова Е. Б.; E. B. Rybkina; Рыбкина Е. Б.; O. V. Seliverstova; Селиверстова О. В.; N. N. Tupitsin; Тупицин Н. Н.; G. I. Uleyskaya; Улейская Г. И.; N. T. Urazalieva; Уразалиева Н. Т.; L. G. Fechina; Фечина Л. Г.; O. V. Khoroshikh; Хороших О. В.; O. E. Tsareva; Царева О. Е.; L. A. Shchekina; Щекина Л. А.; S. A. Plyasunova; Плясунова С. А.; S. A. Lugovskaya; Луговская С. А.

doi:10.17650/1818-8346-2016-11-3-68-75

Results of external quality control study in flow cytometric acute lymphoblastic leukemia diagnostics

Authors: Popov A.M.¹, Verzhbitskaya T.U.², Zueva E.E.³, Anan’eva O.V.⁴, Babenko E.V.⁵, Baydun L.V.⁶, Belyakova E.A.⁷, Boyakova E.V.⁸, Bortnikova O.G.⁹, Gorchakova M.V.¹⁰, Grivtsova L.Y.¹¹, Grinkevich E.N.¹², Davydova Y.V.¹², Zakhar’ko E.I.¹³, Illarionova O.I.¹, Kaprano N.M.¹⁴, Koroleva E.A.¹⁵, Kochengina S.A.¹⁶, Kuz’mina E.G.¹⁷, Kustova E.A.¹⁸, Makarova T.A.¹⁹, Mirolyubova Y.V.²⁰, Murashkina O.E.²¹, Mushkarina T.Y.²², Nisheva E.S.¹⁹, Novikova I.A.²³, Osipova E.Y.¹, Pluzhnikova G.E.⁹, Pochtar M.E.²⁴, Pronkina N.V.²⁵, Rusanova E.V.¹⁰, Rybkina E.B.¹³, Seliverstova O.V.⁸, Tupitsin N.N.¹¹, Uleyskaya G.I.²⁶, Urazalieva N.T.¹⁸, Fechina L.G.², Khoroshikh O.V.²⁷, Tsareva O.E.²⁸, Shchekina L.A.²⁶, Plyasunova S.A.¹, Lugovskaya S.A.²⁴
Affiliations:
1. Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Ministry of Health of Russia
2. Regional Children Clinical Hospital No 1
3. Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
4. Tyumen State Medical University, Ministry of Health of Russia
5. I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, R. M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantology
6. Russian Children Clinical Hospital, Ministry of Health of Russia
7. North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of Russia
8. Blood Transfusion Station of the Moscow Healthcare Department
9. Regional Children Clinical Hospital
10. Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia
11. N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia
12. Nizhnevartovsk Regional Children Clinical Hospital
13. Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia
14. Scientific Clinical Laboratory of Immunophenotyping of Blood Cells and Bone Marrow, National Research Center for Hematology, Ministry of Health of Russia
15. Diagnostic Center of the Altai Region
16. Chelyabinsk Regional Children Clinical Hospital
17. A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia
18. Research Center of Pediatrics and Pediatric Surgery
19. Children City Hospital No 1
20. ФГБУ «Северо-Западный федеральный медицинский исследовательский центр» Минздрава России
21. Children Regional Clinical Hospital
22. A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia
23. Rostov Research Institute of Oncology, Ministry of Health of Russia
24. Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
25. Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
26. City Clinical Hospital No 31
27. Irkutsk the Badge of Honor Order Regional Clinical Hospital
28. Saratov State Medical University named after V.I. Razumovsky, Ministry of Health of Russia
Issue: Vol 11, No 3 (2016)
Pages: 68-75
Section: HEMATOLOGIC MALIGNANCIES: DIAGNOSIS, TREATMENT, SUPPORTIVE CARE
Published: 10.10.2016
URL: https://oncohematology.abvpress.ru/ongm/article/view/208
DOI: https://doi.org/10.17650/1818-8346-2016-11-3-68-75
ID: 208

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Abstract

Comparison of interpretation of acute lymphoblastic leukemia (ALL) flow cytometric diagnostics data was the aim of the study. Immunophenotyping data obtained from 10 patients with ALL were analysed separately in 26 laboratories from Russian Federation and Kazahstan. Results comparison showed four main type of discordance: B-lineage ALL diagnostics during heavy bone marrow regeneration, great variability of T-ALL interpretation, complexity of ambiguous lineage acute leukemia and, finally, very different report types, unique for each laboratory. All these problems are the serious obstacles for standardization of flow cytometric ALL diagnostics in multicenter setting. Continuation of similar QC rounds following by consecutive discussions with further development of consensus diagnostic algorithm could be the first step for standardization of ALL immunophenotyping in Russian Federation and CIS countries.

Keywords

flow cytometry, acute lymphoblastic leukemia, standardization

About the authors

A. M. Popov

Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia

Author for correspondence.
Email: uralcytometry@gmail.com
Russian Federation

T. U. Verzhbitskaya

Regional Children Clinical Hospital No 1

Russian Federation

E. E. Zueva

Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg
State Medical University, Ministry of Health of Russia

кафедра молекулярной биологии, факультет естественных наук Ариэльского университета, лаборатория клинической иммунологии и молекулярной диагностики отдела лабораторной диагностики ГБОУ ВПО «Первый
Санкт-Петербургский государственный медицинский университет им. академика И. П. Павлова» Минздрава России Russian Federation

O. V. Anan’eva

Tyumen State Medical University, Ministry of Health of Russia

Russian Federation

E. V. Babenko

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, R. M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantology

Russian Federation

L. V. Baydun

Russian Children Clinical Hospital, Ministry of Health of Russia

Russian Federation

E. A. Belyakova

North-Western State Medical University named after I.I. Mechnikov, Ministry of Health of Russia

Russian Federation

E. V. Boyakova

Blood Transfusion Station of the Moscow Healthcare Department

Russian Federation

O. G. Bortnikova

Regional Children Clinical Hospital

Russian Federation

M. V. Gorchakova

Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia

Russian Federation

L. Yu. Grivtsova

N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia

Russian Federation

E. N. Grinkevich

Nizhnevartovsk Regional Children Clinical Hospital

Russian Federation

Yu. V. Davydova

Nizhnevartovsk Regional Children Clinical Hospital

Russian Federation

E. I. Zakhar’ko

Central Clinical Diagnostic Laboratory with a Group of Hemostasis Disorders, National Research Center for Hematology, Ministry of Health of Russia

Russian Federation

O. I. Illarionova

Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia

Russian Federation

N. M. Kaprano

Scientific Clinical Laboratory of Immunophenotyping of Blood Cells and Bone Marrow, National Research Center for Hematology, Ministry of Health of Russia

Russian Federation

E. A. Koroleva

Diagnostic Center of the Altai Region

Russian Federation

S. A. Kochengina

Chelyabinsk Regional Children Clinical Hospital

Russian Federation

E. G. Kuz’mina

A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center, Ministry of Health of Russia

Russian Federation

E. A. Kustova

Research Center of Pediatrics and Pediatric Surgery

Kazakhstan

T. A. Makarova

Children City Hospital No 1

Russian Federation

Yu. V. Mirolyubova

ФГБУ «Северо-Западный федеральный медицинский исследовательский центр» Минздрава России

Russian Federation

O. E. Murashkina

Children Regional Clinical Hospital

Russian Federation

T. Yu. Mushkarina

A.F. Tsyb Medical Radiological Research Center – Branch of the National Medical Radiological Research Center,
Ministry of Health of Russia

Russian Federation

E. S. Nisheva

Children City Hospital No 1

Russian Federation

I. A. Novikova

Rostov Research Institute of Oncology, Ministry of Health of Russia

Russian Federation

E. Yu. Osipova

Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev,
Ministry of Health of Russia

Russian Federation

G. E. Pluzhnikova

Regional Children Clinical Hospital

Russian Federation

M. E. Pochtar

Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia

Russian Federation

N. V. Pronkina

Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology

Russian Federation

E. V. Rusanova

Laboratory of Clinical Immunology and Molecular Diagnostics, Department of Laboratory Diagnostics, I. P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia

Russian Federation