Email this article 
Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias
- Authors: Tsaur G.A.1,2,3,4, Popov A.M.5,6, Fechina L.G.1,7, Rumyantsev S.A.5,8,9
-
Affiliations:
- Regional Children Clinical Hospital No 1
- 32 S. Deryabinoy St., Ekaterinburg, 620149, Russia Institute of Medical Cell Technologies
- 22a Karla Marksa St., Ekaterinburg, 620026, Russia Ural Federal University named after the first President of Russia B. N. Yel’tsin
- 19 Mira St., Ekaterinburg, 620002, Russia
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia
- 1 Samory Mashela St., Moscow, 117998, Russia
- 32 S. Deryabinoy St., Ekaterinburg, 620149, Russia
- 1 Samory Mashela St., Moscow, 117998, Russia N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia
- 1 Ostrovityanova St., Moscow, 117997, Russia
- Issue: Vol 11, No 1 (2016)
- Pages: 62-74
- Section: BASIC RESEARCH
- Published: 31.03.2016
- URL: https://oncohematology.abvpress.ru/ongm/article/view/184
- DOI: https://doi.org/10.17650/1818-8346-2016-11-1-62-74
- ID: 184
Cite item
Full Text
Abstract
Hereby we present methodological aspects and prognostic significance of minimal residual disease (MRD) monitoring in infant acute leukemias. Based on our own experience we made algorithm for detection of MRD in this group of patients. We conclude that general concordance between MRD detection by flow cytometry and real-time polymerase chain reaction (PCR) for fusion gene transcripts achieved 87.0 %. Concordance was significantly lower during induction in comparison to consolidation/intensification and relapse treatment (78.6; 90.4 and 93.4 %, correspondingly; p = 0.002). It was not dependent on presence of normal B-cell precursors. Concordance between MRD results obtained by qualitative real-time PCR in bone marrow and peripheral blood samples was 84.5 %. Interestingly, all discrepant results (22 samples 15.5 %) were MRD-positive in bone marrow, but negative in peripheral blood. Despite high qualitative concordance rate between MRD detection in bone marrow and peripheral blood samples we could not show prognostic value of MRD monitoring in peripheral blood by fusion gene transcripts. Multivariate analysis revealed that MRD-positivity at time-point 4 in bone marrow was the only significant and independent prognostic factor of unfavorable outcome in the observed group of patients (hazard ratio 7.326; 95 % confidence interval 2.378–22.565).
About the authors
G. A. Tsaur
Regional Children Clinical Hospital No 1; 32 S. Deryabinoy St., Ekaterinburg, 620149, RussiaInstitute of Medical Cell Technologies; 22a Karla Marksa St., Ekaterinburg, 620026, Russia
Ural Federal University named after the first President of Russia B. N. Yel’tsin; 19 Mira St., Ekaterinburg, 620002, Russia
Author for correspondence.
Email: tsaur@mail.ru
Russian Federation
A. M. Popov
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117998, RussiaRussian Federation
L. G. Fechina
Regional Children Clinical Hospital No 1; 32 S. Deryabinoy St., Ekaterinburg, 620149, RussiaRussian Federation
S. A. Rumyantsev
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117998, RussiaN.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia; 1 Ostrovityanova St., Moscow, 117997, Russia
Russian Federation
References
Supplementary files
