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Cell-binding microarray application in diagnosis of hairy cell leukemia
- Authors: Khvastunova A.N.1,2,3, Al-Radi L.S.4,5, Kapranov N.M.6,7,5, Fedyanina O.S.6,3, Gorgidze L.A.4,5, Lugovskaya S.A.8,9, Naumova E.V.8,9, Dzhulakyan U.L.4,5, Filatov A.V.10,11, Ataullakhanov F.I.1,2,7,5, Kuznetsova S.A.1,2,7,5
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Affiliations:
- Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia
- 1 Samory Mashela St., Moscow, 117198, Russia Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences
- 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia
- Hematological Research Center, Ministry of Health of Russia
- 4a Novo-Zykovskiy Pr-d, Moscow, 125167, Russia
- Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences
- 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia Hematological Research Center, Ministry of Health of Russia
- Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia
- 2/1 Barrikadnaya St., Moscow, 125993, Russia
- State Research Center “Institute of Immunology”, Federal Medical and Biological Agency, Moscow
- 24, Bldg. 2 Kashirskoe Shosse, Moscow, 115478, Russia
- Issue: Vol 10, No 1 (2015)
- Pages: 37-45
- Section: BASIC RESEARCH
- Published: 04.06.2015
- URL: https://oncohematology.abvpress.ru/ongm/article/view/137
- DOI: https://doi.org/10.17650/1818-8346-2015-1-37-45
- ID: 137
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Abstract
We describe an application of a cell-binding microarray – to parallel study of morphology, tartrate-resistant acid phosphatase activity and detection of surface markers on peripheral blood lymphocytes of 90 atients with suspected hairy cell leukemia (HCL). We have formulated the microarray-based diagnostic criteria for hairy cell leukemia, hairy cell leukemia variant (HCLv) and splenic marginal zone lymphoma (SMZL). According to these criteria we have suggested the presence of HCL for 55 patients, HCLv – for 7 patients and SMZL – for 10 patients from the studied cohort. These diagnoses were confirmed by standard diagnostic methods in all cases. These results show that the cellbinding microarray can be used in differential diagnosis of HCL, while the high sensitivity of the microarray permits to detect the leukemic cells in spite of leukopenia and low hairy cell content.
About the authors
A. N. Khvastunova
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, RussiaTheoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia
Author for correspondence.
Russian Federation
L. S. Al-Radi
Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaRussian Federation
N. M. Kapranov
Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, RussiaHematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, Russia
Russian Federation
O. S. Fedyanina
Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, RussiaRussian Federation
L. A. Gorgidze
Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaRussian Federation
S. A. Lugovskaya
Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia; 2/1 Barrikadnaya St., Moscow, 125993, RussiaRussian Federation
E. V. Naumova
Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia; 2/1 Barrikadnaya St., Moscow, 125993, RussiaRussian Federation
U. L. Dzhulakyan
Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaRussian Federation
A. V. Filatov
State Research Center “Institute of Immunology”, Federal Medical and Biological Agency, Moscow; 24, Bldg. 2 Kashirskoe Shosse, Moscow, 115478, RussiaRussian Federation
F. I. Ataullakhanov
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, RussiaTheoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia
Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, Russia
Russian Federation
S. A. Kuznetsova
Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, RussiaTheoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia
Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, Russia
Russian Federation
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