Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia

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Abstract

   Waldenstrom macroglobulinemia is a lymphoplasmacytic lymphoma, the morphological substrates of which are b‑lymphocytes, proplasmocytes, and plasma cells. The world Health Organization recommends multicolor flow cytometry with analysis of markers such as IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103, Cd138, for diagnosing this disease. Based on international and our own experience, we recommend that tumor b‑lymphocytes and plasma cells be analyzed separately for the diagnosis of waldenstrom macroglobulinemia, since the immunophenotypic profile of these populations differs. In diagnostics, this approach provides a more complete understanding of various subpopulations contribution, and when monitoring minimal residual disease, it helps to detect the tumor clone, which after therapy is predominantly represented by plasma cells. we recommend using antibodies to surface and intracellular markers such as Cd138, Cd38, Cd19, Cd45, Cd20, Cd22, Cd27 cytκ, cytλ and cytIgM for immunophenotypic testing of waldenstrom macroglobulinemia.

About the authors

I. V. Galtseva

National Medical Research Center for Hematology, Ministry of Health of Russia

Author for correspondence.
Email: galtseva.i@blood.ru
ORCID iD: 0000-0002-8490-6066

Irina Vladimirovna Glatseva

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

Yu. A. Tsoy

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0009-0005-7828-1556

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

A. E. Grachev

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0003-4950-523X

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

N. M. Kapranov

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0002-6512-910X

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

K. A. Nikiforova

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0002-4119-7175

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

Yu. O. Davydova

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0001-5932-0285

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

A. A. Kulikov

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0009-0001-0544-6568

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

E. E. Zvonkov

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0002-2639-7419

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

E. N. Parovichnikova

National Medical Research Center for Hematology, Ministry of Health of Russia

ORCID iD: 0000-0001-6177-3566

125167; 4 Novyy Zykovskiy Proezd; Moscow

Russian Federation

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77-36928 от  21.07.2009.